Determination of silymarin in microemulsion by RP-HPLC

Abstract

Abstract:

We established a RP-HPLC method for the analysis of silymarin in microemulsion. Silymarin was separated using a ODS C18 column and monitored at the wavelength 288 nm. The mobile phase consisting of methanol-water-acetic acid (42:58:0.5, v/v/v) was pumped at a flow rate of 1.0 mL/min. The linear range of calibration curve was 10-1000 μg/mL. The average recovery was 99.0%-100.7% for silybin isomers. The RSD values of inter-day and intra-day assays were lower than 1.6% for silybin isomers. The method is simple, rapid, reproducible and precise for the quantitative determination of silymarin in microemulsion.

Key words: Silymarin, Silymarin, Silybin, Silybin, Microemulsion, Microemulsion, RP-HPLC, RP-HPLC

CLC Number:

R917.1

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Xin-Ru Li, Yan-Fang Li, Yan-Xia Zhou, Xiao-Yan Li, Yan Liu^*. Determination of silymarin in microemulsion by RP-HPLC[J]. .

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URL: http://jcps.bjmu.edu.cn/EN/

http://jcps.bjmu.edu.cn/EN/Y2009/V18/I2/190

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