Abstract: Two triterpenoid saponins (compound I and II) have been isolated from Gymnocladus chinensis Baill., and compound I was determined as a new compound. The structure of compound I was assigned as 2β,23-dihydroxy-acacic acid-3-O-α-L-arabinopyranosyl-21-O-{(6S)-2-E-2,6-dimethyl-6-O-[4-O-(6S)-2-E-2,6-dimethyl-6-O-β-D-glucopyranosyl-α-L-arabinopyranosyl-2,7-octadienoyl}-28-O-β-D-xylopyranosyl(1→3)-β-D-xylopyranosyl(1→4)-α-L-rhamnopyranosyl(1→2)-[α-L-rhamnopyranosyl-(1→6)]-β-D-glucopyranoside by extensive MS and NMR studies.

Key words: 1D/2D NMR, 1D/2D NMR, Triterpenoid saponins, Triterpenoid saponins, Gymnocladus chinensis Baill., Gymnocladus chinensis Baill.