Abstract: To investigate the absorption of total triterpenoids from Ganoderma lucidum in rats. HPLC-DAD and LC-MS methods were used to identify ganoderic acids in rat plasma after oral administration of total triterpenoids from G. lucidum by comparing their HPLC retention behaviors, UV absorption spectra, and mass spectra with authentic samples. Five ganoderic acids, ganoderic acid C2, C6, G, B and A were simultaneously detected in rat plasma. Ganoderic acids can be directly absorbed into circulation after oral administration of total triterpenoids from G. lucidum in rats.

Key words: Ganoderma lucidum, Ganoderma lucidum, Triterpenoids, Triterpenoids, Ganoderic acids, Ganoderic acids, LC-MS, LC-MS, HPLC-DAD, HPLC-DAD, ,