Analysis of principal isoflavone glycosides and aglycones in Radix astragali

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Analysis of principal isoflavone glycosides and aglycones in Radix astragali

Xin-Gang Du, Yan-Jing Bai, Bin Wang, Yu-Ying Zhao, Qing-Ying Zhang^*, Lu-Qi Huang

1. The State Key Laboratory of Natural and Biomimetic Drugs;
Department of Natural Medicines, School of Pharmaceutical Sciences, Peking University, Beijing 100191, China;
2. Institute of Chinese Materia Medica, China Academy of Traditional Chinese Medicine, Beijing 100700, China

Received:2008-03-15 Revised:2008-08-10 Online:2008-09-15 Published:2008-09-15
Contact: Qing-Ying Zhang*

Abstract

Abstract:

Two major isoflavone glycosides [calycosin 7-O-β-D-glucopyranoside (1) and ononin (2)] and their aglycones [calycosin (3) and formononetin (4)] were simultaneously quantified with HPLC/DAD method. Two unknown compounds were identified as calycosin 7-O-β-D-glucopyranoside-6'''-O-malonate (U1) and formononetin 7-O-β-D-glucopyranoside-6'''-O-malonate (U2), respectively, with LC/MSn. Raw Radix astragli were shown to have higher contents of isoflavone glycosides (1, 2), but lower contents of aglycones (3, 4) than the processed herbal materials. After being moistened with water and stored up for 24 h at 35 ºC, the glycosides and their malonates were almost completely transformed to their corresponding aglycones. The different contents of the isoflavone glycosides and their aglycones in raw and processed Radix astragali materials might be due to enzymolysis of the glycosides during processing.

Key words: Radix astragali, Radix astragali, Astragalus membranaceus var mongholicus, Astragalus membranaceus var mongholicus, Astragalus membranaceus, Astragalus membranaceus, Isoflavones, Isoflavones, ,

Supporting:

Xin-Gang Du, Yan-Jing Bai, Bin Wang, Yu-Ying Zhao, Qing-Ying Zhang^*, Lu-Qi Huang. Analysis of principal isoflavone glycosides and aglycones in Radix astragali
[J]. .

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