RP-HPLC determination of lycopene in microcapsules

Abstract

Abstract: Aim A RP-HPLC method for determination of lycopene in microcapsules was established. Methods The HPLC assay was performed on an Alltima C₁₈(4.6 mm × 250 mm, 5 μm) column with a mixture of methanol-THF-water (66:30:4, V/V/V) as mobile phase at a flow rate of 1.5 mL·min^-1 and the UV detection wavelength was 472 nm. Results The linear range of lycopene was 3.6-18 μg·mL^-1, r = 0.9998, the average recovery was from 99.81% to 101.06% with RSD less than 1.83%. The RSD of intra-day and inter-day precision were less than 3.34%. Conclusion The method is simple, accurate and suitable for the determination of lycopene in microcapsules.

Key words: RP-HPLC, RP-HPLC, Lycopene, Lycopene, Microcapsules, Microcapsules

CLC Number:

Supporting:

Ding-Qiang Lu^*, Xiao-Yun Lu, Kai-Feng Lu, Ji-Jun Hu . RP-HPLC determination of lycopene in microcapsules[J]. .

References

[1] Neuyen ML, Schwartz SJ. Lycopene: chemical and properties [J]. Food Technol (Chicago), 1999, 539 (2): 38–54.
[2]Giovannucci E, Ascherio A, Rim, EB, et al. Intake of carotenoids and retinol in relation to risk of prostate cancer [J]. J Natl Cancer Inst, 1995, 87 (23): 1767–1775.
[3] Clinton SK. Lycopene: chemistry, biology, and implications for human health and disease [J]. Nutr Rev, 1998, 56: 35–51.
[4] Giovannucci E. Tomatoes, tomato-based products, lycopene, and cancer: review of the epidemiologic literature [J]. J Natl Cancer Inst, 1999, 91: 317–331.
[5] Huang Y, Xia QY, Liu SX, et al. Research advance and application of lycopene [J]. South China Univ Trop Agri, 2006, 12 (2): 50–55.
[6] Sun QJ, Ding XL. The studies on the stability of lycopene [J]. J Food Ferment Ind, 1998, 24 (2): 47–49.
[7] Zuo, AR, Fan QS, Liu Y, et al. Study on technique of microencapsulation of lycopene [J]. J Food Sci, 2004, 25 (4): 35–39.
[8] Fang JY, Yu CY, Yao H. Preparation of lycopene microcapsule by spray-drying [J]. Drying Technol Equip, 2005, 3 (1): 19–23.
[9]Wang, YS, Yuan QP, Qin JG, et al. Effect of the lowmolecular modulator on lycopene fermentation [J]. Food Sci, 2003, 24 (5): 97–100.
[10] Froescheis O, Moalli S, Liechti H, et al. Determination of lycopene in tissues and plasma of rats by normal- phase high-performance liquid chromatography with photometric detection [J]. J Chromatogr B, 2000, 739 (2): 291–299.
[11] Tzouganaki ZD, AttaPolitou J, Koupparis MA. Development and validation of liquid chromatographic method for the determination of lycopene in plasma [J]. Anal Chim Acta, 2002, 467: 115–123.
[12] Careri M, Elviri L, Mangia A. Liquid chromatography-electrospray mass spectrometry of β-carotene and xanthophylls validation of the analytical method [J]. J Chromatogr A, 1999, 854: 233–244.
[13] Qi, GP, Zhao, SQ. Analysis of lycopene by supercritical fluid chromatography [J]. Chin J Anal Chem, 2002, 30 (12): 1477–1480.
[14] Anocha K. Analysis of carotenoid [J]. Food Chem, 1998, 63 (4): 577–584.

[1]	Xiuwei Yang, Youbo Zhang, Wei Xu. Determination and pharmacokinetic study of p-hydroxyphenethyl anisate following intravenous and oral administration in rats by RP-HPLC method [J]. Journal of Chinese Pharmaceutical Sciences, 2020, 29(11): 804-812.
[2]	Wenjuan OuYang, Yalan Zhang, Pingsheng Xu, Zhiyong Dai, Hongying Ma, Qun Qin. Pharmacokinetics of Pazufloxacin Mesilate Sodium Chloride in healthy Chinese volunteers after intravenous injection [J]. Journal of Chinese Pharmaceutical Sciences, 2019, 28(1): 40-48.
[3]	Agha Zeeshan Mirza. HPLC-UV method for simultaneous determination of irbesartan, candesartan, gliquidone and pioglitazone in formulations and in human serum [J]. Journal of Chinese Pharmaceutical Sciences, 2018, 27(4): 273-280.
[4]	Yuan Zhou, Ning Li, Jiayu Zhang, Yong Jiang, Pengfei Tu. Simultaneous determination of four triterpenoid saponins and two liganoids in the roots of Ilex pubescens by RP-HPLC-DAD [J]. Journal of Chinese Pharmaceutical Sciences, 2014, 23(12): 866-872.
[5]	Prajapati Prajesh^*, Vaghela Vipul, Baraiya Dharmendra. Sensitive method for rapid estimation of Lornoxicam in bulk and its dosage form by RP-HPLC [J]. , 2013, 22(1): 110-113.
[6]	Ling Zhang, Yan-Ping Zhao, Hua Qiao, Ting Wang^*, Li Liang, Wei Chang, Dan Li, Pei-Han Xu . Quantitative determination of nizatidine in human plasma and urine by high performance liquid chromatography and its pharmacokinetic study in humans [J]. , 2010, 19(4): 279-284.
[7]	Ying He, Xiao-Juan Chai, Su Zeng^* . Reversed-phase high-performance liquid chromatographic analysis of seven pairs of chiral drug enantiomers in transport medium after chiral derivatization [J]. , 2010, 19(2): 104-109.
[8]	Yan-Li Xu, Qi Dong, Feng-Zu Hu^*. Simultaneous quantitative determination of eight active components in Polygonum multiflorum Thunb by RP-HPLC [J]. , 2009, 18(4): 358-361.
[9]	Xin-Ru Li, Yan-Fang Li, Yan-Xia Zhou, Xiao-Yan Li, Yan Liu^*. Determination of silymarin in microemulsion by RP-HPLC [J]. , 2009, 18(2): 190-192.
[10]	Yan-Bin Shi^*, Yan-Ping Shi,Xiao-Yun Zhang,Quan-Yi Zhao,Jing-Man Ni . Determination of bergenin in rat urine, feces and tissues by RP-HPLC method [J]. , 2009, 18(1): 49-54.
[11]	Xiang-Rui Liu, Ke-Chun Wu, Chun-Hui Zhang, Xuan Zhang^*, Qiang Zhang. A RP-HPLC method for determination of paclitaxel in its solid dispersion [J]. , 2007, 16(2): 101-104.
[12]	NIU Feng, XIE Guang-bo, CUI Zheng, CHEN Fa-kui, TU Peng-fei^. Determination of Griffonilide in Roots of Semiaquilegia adoxoides* by RP-HPLC [J]. , 2006, 15(3): 168-171.
[13]	DENG Yi-hui^*, WU Hong-bing, LU Yi, ZHOU Xin-yu, WANG Ning, ZHAO Jing. Determination of 1-Phenylpropanol in Raw Material and Preparations by High-Performance Liquid Chromatograph [J]. , 2006, 15(1): 45-50.
[14]	CHEN Jia, SONG Shao-jiang^*, SONG Ning. Simultaneous Determination of 2''-O-rhamnosyl Vitexin and Vitexin in Chinese Hawthorn Leaf and Its Extract by RP-HPLC [J]. , 2006, 15(1): 51-54.
[15]	MA Lei, YIN Li-li, SUN Jin, HE Zhong-gui^*. A Sensitive High-Performance Liquid Chromatography Coupled with Solid- Phase Extraction for Determination of Fexofenadine in Beagle Plasma [J]. , 2005, 14(4): 246-249.