Abstract: Aim To investigate the electrochemical behaviors of Mitomycin C (MC) and its interaction with calf thymus DNA (ctDNA). Methods The cyclic voltammetry (CV) was carried out at a paraffined graphite electrode. Results MC showed a well-defined oxidation-reduction peak. As a result of reaction with ctDNA, the peak current of MC decreased apparently. According to corresponding electrochemical equations, the diffusion coefficient of both free and MC-DNA complex have been determined, and the heterogeneous rate constants were also obtained simultaneously. Conclusion The solid paraffined graphite electrode could be used to estimate parameters of the interaction between DNA and MC, and provide the convenient and sensitive analysis.

Key words: mitomycin C, mitomycin C, calf thymus DNA, calf thymus DNA, cyclic voltammetry, cyclic voltammetry, paraffined graphite electrode, paraffined graphite electrode