Preparation of BCEC-Astrocyte Co-culturing Membrane Plate Insert

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Preparation of BCEC-Astrocyte Co-culturing Membrane Plate Insert

ZHAO Kang-feng, WANG Qi, PU Xiao-ping^*, YANG Xiu-wei, ZHU Yu-zhen

1.Department of Toxicology, Lanzhou Medical College, Lanzhou 730000, China;
2.Department of Molecular and Cellular Pharmacology, School of Pharmaceutical Sciences, Peking University, Beijing 100083, China;
3.State Key Laboratory of Natural and Biomimetic Drugs, Peking University, Beijing 100083, China

Received:2004-03-31 Revised:2004-11-10 Online:2004-12-15 Published:2004-12-15
Contact: PU Xiao-ping*

Abstract

Abstract: Aim To prepare a hand-made micropore membrane culture plate insert for co-culture. Methods The plate insert was made using plastic centrifuge tube and micropore membrane. After seeding brain capillary endothelial cells (BCECs) on it (under the effect of astrocyte-conditioned medium), the plate insert was assessed by analysis of trans-endothelial electrical resistance (TEER). Results The plate insert has a stability of at least 15 d under culture condition. TEER increased significantly under co-culture condition from (66.1±13.3) Ωcm² to (182.2±6.7) Ωcm². Conclusion This micropore membrane culture plate insert can be easily made, on which BCEC culture can be successfully performed. Moreover, it is adjustable and recyclable. It follows that the plate insert is a useful tool for co-culture and the related research fields.

Key words: blood-brain barrier, blood-brain barrier, culture plate insert, culture plate insert, brain capillary endothelial cell, brain capillary endothelial cell, astrocyte, astrocyte, trans-endothelial electronic resistance, trans-endothelial electronic resistance

CLC Number:

R965.2

Supporting:

ZHAO Kang-feng, WANG Qi, PU Xiao-ping^*, YANG Xiu-wei, ZHU Yu-zhen. Preparation of BCEC-Astrocyte Co-culturing Membrane Plate Insert[J]. .

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