Abstract: Aim A method for determination of lycopene concentration in dog plasma was established. Methods RP-HPLC was used; the mobile phase wavelength of detection was 472 nm, the column temperature was ambient temperature, and the flow rate was 1.0 mL·min^-1. Results The standard curve was linear in the range from 0.012 4 to 0.496 μg·mL^-1 with r = 0.9992. The average extraction recovery was 97.6%±4.2%. The intra-day and inter-day RSD were 1.52%-4.95% and 2.31%-7.38%, respectively. Conclusion This method is sensitive, rapid, reproducible, and of good selectivity for the analysis of lycopene in dog plasma.

Key words: lycopene, lycopene, plasma concentration, plasma concentration, RP-HPLC, RP-HPLC