Abstract: Aim To study isolation and purification of Gonyautoxins from two different strains of Alexandrium minutum Halim. Methods Ethanol extracts of the two culture strains of Alexandrium minutum Halim were fractionated by gel filtration and ion exchange chromatography to obtain gonyautoxins. The toxin profiles were tested by HPLC method. Results Both strains contain gonyautoxin-1, gonyautoxin-2, gonyautoxin-3 and gonyautoxin-4. The total gonyautoxin content of Amtk4 strain is almost 10 times higher than that of Amtk2 strain. Conclusion Alexandrium minutum Halim Amtk4 strain is suitable for the preparation of Gonyautoxins.

Key words: Alexandrium minutum Halim, Alexandrium minutum Halim, gonyautoxins, gonyautoxins, gel filtration chromatography, gel filtration chromatography, ion exchange chromatography, ion exchange chromatography