Abstract: Aim To establish an RP-HPLC method for the determination of scutellarin in plasma and study its pharmacokinetics in dogs. Methods Scutellarin was given to dogs by intravenous injection and determined by RP-HPLC, the mean plasma concentration-time curve was plotted and pharmacokinetic parameters were calculated by program 3p87. Results The concentration-time curve of scutellarin could be fitted to three-compartment model with T1/2pi, T1/2α and T1/2β being 1.05±0.80 min, 6.99±2.76 min and 51.61±28.78 min, respectively, Vc being 880.1±508.3 mL, CL being 189.6±53.8 mL·min^-1, and AUC0-90 and AUC0-∞ being 574.43±133.95 μg·min·mL^-1 and 599.34±132.00μg·min·mL^-1, respectively. Conclusion The fact that the concentrations of scutellarin in plasma declined rapidly after the medication suggested that the T1/2 of scutellarin should be taken into account in drug administration and preparation development.

Key words: scutellarin, scutellarin, pharmacokinetics, pharmacokinetics, RP HPLC, RP HPLC