Abstract: A useful helicobacter pylori-induced gastritis model using BALB/c mice was established for mimicking of human gastritis induced by Helicobacter pylori (H. pylori). The H. pylori isolates were obtained freshly from a human complexulcer patient. BALB/c mice were fasted for 24 h and then 0.25 mL of 0.2 mol·L^-1 NaHCO3 was administered after by gavageto each mouse and 0.5 mL of 109 colonies formation unit per milliliter (CFU/mL) of H. pylori was administered 15 min. Onthe 3^rd day and 5^th day, the H. pylori inoculations were repeated. The inoculated mice were sacrificed in batch on the 5^th day, in the 2^nd week, 3^rd week and 4^th week. The gastric mucous membrane near pyloric portion was removed, treated and then cultured under microaerobic condition for detection of H.pylori. The remainders of the gastric membrane were fixed by 10% formaldehyde solution for pathological detection. The results showed that the H. pylori could be separated from the gastric membranes of inoculated mice. Obvious invasion of inflammatory cells in the gastric membranes of inoculated mice could be ob-served from pathologieal sections. It can be concluded that the inoculating fresh human H. pylori isolates can produce mousegastritis. This model of BALB/c mice can be used for evaluating the therapeutic agents for the treatment of gastritis induced by H. pylori.

Key words: Helicobacter pylori, Helicobacter pylori, Gastritis , Gastritis , BALB/c mice, BALB/c mice