Abstract: China has a great resource of Cannabis. Research on the taxonomy and morphology of Chinese Cannabis has been carried out, but so far no molecular genetic research has been published. Random amplified polymorphic DNA (RAPD) is a suitable technique for molecular genetic research on Cannabis. In this experiment, using Cannabis herbarium specimens as a source of genetic materials, the correlative conditions of the Polymerase chain reaction (PCR), (i.e., gradient density of Mg²⁺, dNTPs, Taq DNA polymerase, anneal temperature, anneal time and reaction cycles) were examined separately. An effective procedure for the RAPD analysis of Cannabis was obtained.

Key words: Cannabis, Cannabis, Herbarium specimen, Herbarium specimen, Random amplified polymorphic DNA, RAPD, Random amplified polymorphic DNA, RAPD