Abstract: Effect of folio acid on delivery of liposomes was examined using cultured HeLa cells. Folateliposomes encapsulating calcein and liposomes were cultured with HeLa cells respectively. Liposomes taken up bycells were measured by a fluorescence spectrophotometer. The cell fluorescence increased slowly at the 4 h timepoint, and the saturated folate-liposome concentration was 0.45 mg·mL^-1. The binding of folate-liposomes to HeLacells could be competitively inhibited by excess free folate. Enzyme phospholipase D (PLD) or phosphatidylinositolspecific phospholipase C (PIPLC) influenced liposome uptake via folate receptor. Folate targeting improved the specificity of liposomes for cells which overexpressed the folate receptors.

Key words: Folate receptor, Folate-liposome, Folate receptor, Folate-liposome, HeLa cells, HeLa cells, Target, Target