Abstract: Ebselen. 2-phenyl-1,2-benzisoselenazol-3 (2H)-one, activated by. glutathione(GSH) was shown to react with N-acetyl-p-benzoquinone imine (NAPQI). the toxic rnetabolite ofparacetamol, but not to react with paracetamol. The GSH-activaled ebselen reacts uith NAPQI fasterand more complete than with ebselen alone. Compared xvith the UV-spectra of the solution containing20 μmol/L ebselen tactivated by 20 μmol/L GSH) and 20 μmol/L NAPQI with the solution contain-ing 20 μmol/L ebselen tactivated by 20 μmol/LGSH) and 20 μmol/L paracetamol, NAPQI was foundto be reduced to paracetamol by activated ebselen. The optimal PH range for this reaction was foundto be 7.4 to 8.2.

Key words: Ebselen: Glutathione, NAPQI, paracetamol