Abstract: The interaction between cisplatin and erythrocyte membrane proteins was studied based on the quenching effect of cisplatin on the intrinsic fluorescence of proteins. A concentration-dependent quenching effect was observed. The presence of chloride and sulphate weakens the effect significantly. A pH-dependence was also noted with a stronger effect in acidic solution. The nature of the interaction is considered to be platinum-thiol group binding according to the effect of cisplatin on the fluorescence of FMA labeled membrane. The mechanism of the cisplatin-protein interactions was discussed based on the effect of coexisting anion.

Key words: Cisplatin, Membrane protein, Fluorescence