Abstract: The Rifampicin (RFP) specific IgG in the sera of rabbits immunized with RFP or with two kinds of RFp-protein conjugates (RFP-bovine serumalbumin, RFP-BSA, and RFP-rabbit serum albumin, RFP-RSA) was measured by ennyme-linked immunosorbent assay (ELISA).The way of conjugation and the molecular cojugation rate were different between RFP-BSdA and RFP-RSA. The titre of the RFP specific IgG was l:l0-l:l00 in RFP immunized rabbits, 1:100-1:1000 in RFP-RSA immunized rabbits and overl:l000 in RFP-BSA immunized rabbits. The binding properties of the sera to RFP were demonstrated by inhibition assay. These are the first RFP sensitization models established by immunizing animals with RFP itself or RFP-protein conjugates.

Key words: Rifampicin, Animalmodel, Hypersensitivity, Enzyme-linked immunosothent assay