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Selective acetylation of puerarin by Rhodococcus sp.

Ya-Guang Ding, De-Wu Zhang, Shu-Min Liu, Jun-Gui Dai*   

  1. 1. State Key Laboratory of Bioactive Substance and Function of Natural Medicines, Institute of Materia Medica, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100050, China
    2. College of Traditional Chinese Medicine, Heilongjiang University of Traditional Chinese Medicine, Haerbin 150040, China
  • Received:2012-02-13 Revised:2012-04-10 Online:2012-05-15 Published:2012-05-15
  • Contact: Jun-Gui Dai*

Abstract:

Among 39 species of microbial strains, Rhodococcus sp. AS 4.1147 possessed the ability to selectively acetylate puerarin (1) at C-6 position of the glucosyl moiety. The structure of the acetylated product, 6''-O-acetylpuerarin (2) was determined by the analysis of MS, NMR spectroscopic data. The isolated yield of 2 was 22.2%.

Key words: Puerarin, Selective acetylation, Rhodococcus sp. AS 4.1147

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