Simultaneous determination of five nucleosides and nucleobases in Panax notoginseng using high-performance liquid chromatography

Abstract

Abstract: Aim To quantitatively determine five nucleosides and nucleobases, including cytidine, uridine, guanosine, adenosine and uracil in different parts of Panax notoginseng. Methods Separation was performed on a Zorbax SB-Aq column using a gradient elution with mobile phase of 8 mmol·L^–1 ammonium acetate aqueous solution (A) and methanol (B). The assay was carried out at a flow rate of 1 mL·min^–1 at 25 ºC with the diode-array detection at 260 nm. Results Cytidine, uridine, guanosine, adenosine and uracil had good linearity in the ranges of 1.79 – 57.40 μg·mL^–1 (r² = 1.0000), 3.30 – 105.60 μg·mL^–1 (r² = 1.0000), 3.09 – 98.80 μg·mL^–1 (r² = 0.9999), 2.77 – 88.60 μg·mL^–1 (r² = 1.0000) and 0.38 – 12.30 μg·mL^–1 (r² = 1.0000) with average recoveries of 93.9%, 96.5%, 92.7%, 93.2% and 98.8%, respectively. The content of cytidine, uridine, guanosine, adenosine and uracil in different parts of P. notoginseng were significantly different. Conclusion This is the first report on quantitative determination of nucleosides and nucleobases in P. notoginseng.

Key words: Panax notoginseng, Panax notoginseng, Nucleoside, Nucleoside, Nucleobase, Nucleobase, High-performance liquid chromatography HPLC, High-performance liquid chromatography HPLC

CLC Number:

R917

Supporting:

Jing Wang, Yi-Tao Wang, Shao-Ping Li^*. Simultaneous determination of five nucleosides and nucleobases in Panax notoginseng using high-performance liquid chromatography[J]. .

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