Abstract: Microspheres I, II and III were produced by emulsion technique.Microsphere I was solidified by glutaraldehyde crosslinking, microsphere II was solidified by glutaraldehyde crosslinking and further treated with glycine solution and microsphere Illwas solidified by heating denaturation only.The results showed that the microsphere diameter produced by cross[inking was bigger than that prepared by heating.The microsphere II had higher hydrophilicity than Microsphere I had.The methotrexate (MTX) contents in microspheres I and II were 2.73±0.053%, 2.87±0.119% respectively. microsphere III was only blank microspheres with MTX adsorbed on their surfaces.In vitro release studies, microspheres I and I have maintained sustained release of MTX till the next day, it was found that the drug releases from microspheres I and II were governed by Higuchi diffusion law.

Key words: Hydrophillic albumin microspheres, Emulsion technique, Methotrexate, Drug release kinetics