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Methodological Studies on Genomic DNA Extraction and Purification from Plant Drug Materials

Hui Cao, Paul Pui-Hay But, Pang-Chui Shaw   

  1. 1.I nstitute of Chinese Materia Medica, China Academy of Traditional Chinese Medicine, Beijing 100700;
    2. Department of Biology, The Chinese University of HongKong, HongKong;
    3. Department of Biochemistry, The Chinese University of HongKong, HongKong;
    4. Chinese Medicinal Material Research Center, The Chinese University of HongKong, HongKong
  • Received:1997-05-16 Revised:1998-04-07 Online:1998-09-15 Published:1998-09-15

Abstract: This paper reports the rationale and methods of DNA extraction and purification from nine species of Compositae and four commercial drugs of corresponding plant Elephantopus scaber. The comparison of three methods: CsCl gradient, CTAB/CsCl gradient and CTAB miniprep extraction by yield, purity and factors affecting PCR was carried out. In conclusion, CTAB miniprep method provides a rapid, effective, economic approach for isolating genomic DNA for Chinese drug identification by genomic fingerprints.

Key words: DNA extraction, DNA purification, CTAB miniprep, Genomic fingerprinting, AP-PCR

Supporting: