Abstract: By techniques of gene clone, microinjection and patch-clamp, human arrhythmicrelated gene Kv1 .5 and Kv4.2 were translated into cRNA, and then injecte4 into Xenopus oocytes, respectively. A pure and single K⁺ current of ultrarapid delayed rectifier K⁺ current (IKur) or transientoutward K⁺ current (Ito) was respectively detected on Xenopus oocytes. This is a modern pharmacological model for evaluating class III antiarrhythmic drugs. It can overcome many defects of previousmethods for evaluating antiarrhythmic drugs, such as lacking human fresh cardiac muscle cells asmaterial, and co-expression of many currents on cell membrane.

Key words: Arrhythmic gene, Kv1.5, Kv4.2, Xenopus oocyte, Pharmacological model