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The effects of pinellia total alkaloids on the amino acid concentration and the GABAA receptor expression in hippocampus region of epileptic rats

Yan-Ting Gu, Yong-Gang Ma, Ming-Zheng Wang*, Xin-Feng Wang, Yi Xiao   

  1. Department of Pharmacology, Shanxi Medical University, Taiyuan 030001, Shanxi, China
  • Received:2009-04-14 Revised:2009-08-10 Online:2009-09-15 Published:2009-09-15
  • Contact: Ming-Zheng Wang*

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Abstract:

The antiepileptic effect of pinellia total alkaloids (PTA) on penicillin (PNC) chronically kindled rats was investigated. We investigated the effects of PTA on Glu, Asp, Gly and γ-aminobutyric acid (GABA) concentrations and the expression level of cerebral GABAA receptor in hippocampus. The influence of PTA on epilepsy seizure latency and degree in PNC chronically kindled rats were observed. High performance liquid chromatography (HPLC) was adopted to measure the concentrations of Glu, Asp, Gly and GABA in hippocampus. Reverse transcriptase-polymerase chain reaction (RT-PCR) was used to determine the expression of cerebral GABAA receptor mRNA. Compared with normal rats, the levels of GABA and Gly decreased obviously while the level of Glu and Asp increased significantly in model rats. The cerebral GABAA receptor mRNA level was also decreased at the same time. The difference was statistically different compared to the control group. PTA could prolong the latent period of the penicillin induced seizure and weaken the extent of seizure, compared with the model group without PTA treatment. Moreover, PTA increased the level of GABA and the expression level of GABAA receptor, while decreased the level of Glu significantly. However, it had no obvious effect on the level of Gly and Asp. Pre-treatment of PTA can also increase the GABAA receptor mRNA level. In conclusion, PTA could alleviate the PNC chronically kindled rat seizure. It increased the GABA level and the expression of GABAA receptor, and it decreased the Glu concentration.

Key words: Pinellia total alkaloids PTA, Pinellia total alkaloids PTA, PNC chronically kindled rats, PNC chronically kindled rats, Anticonvulsant action, Anticonvulsant action, Neurotransmitters, Neurotransmitters, GABAA receptor, GABAA receptor

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