Enhancement of fluorescence of terbium and transferrin-bound diterbium by HEPES

Abstract

Abstract: Terbium (Tb) has been extensively used as a fluorescence probe for the identification of calcium-binding sites in proteins and for fluorometric analysis of organic ligands. In the current study, we reported that HEPES, a commonly used pH buffer reagent, significantly enhanced the characteristic emission of Tb at 585 nm. The maximum emission of Tb at 490 nm and 549 nm were also enhanced by HEPES to a less extent. Thus, cautions should be taken when quantitative analysis is performed based on the fluorescence emission of Tb at 549 nm, since the emission may vary due to the buffer reagents. Additionally, the fluorescence intensity at 585 nm was proportional to the concentration of both HEPES and terbium ions, which might be utilized to develop new fluorometric analytical methods.

Key words: Tb3+, Tb3+, HEPES, HEPES, Fluorescence, Fluorescence, Tb2Tf, Tb2Tf, ApoTf , ApoTf

CLC Number:

R916.03

Supporting:

Ping Du¹, Lan Yuan^2*, Hou-Nan Wu², Ke-Ning Xu³, Xiao-Gai Yang^1*. Enhancement of fluorescence of terbium and transferrin-bound diterbium by HEPES [J]. .

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