Abstract: Aim To establish a capillary electrophoresis method for enantiomeric separation of meptazinol hydrochloride. Methods The separation conditions such as cyclodextrin (CD) type, buffer pH, concentration of 2,3,6-O-trimethyl-β-cyclodextrin and organic additives were optimized. An optimum concentration was 30 mmol·L^-1 phosphate (pH 7.02) with 10% (W/V) TM-β-CD and 2% acetonitrile. Results Baseline resolution of the enantiomer was readily achieved using 2, 3, 6-O-trimethyl-β-cyclodextrin. Conclusion This is a convenient method for fast enantiomeric resolution of meptazinol hydrochloride.

Key words: meptazinol hydrochloride, meptazinol hydrochloride, enantiomeric separation, enantiomeric separation, capillary electrophoresis, capillary electrophoresis