Simultaneous Determination of Ceftazidime and Tazobactam in Injectable Powder by Reversed-Phase High Performance Liquid Chromatography

Abstract

Abstract: Aim A reversed-phase high performance liquid chromatographic (RP-HPLC) method was developed and validated for the simultaneous determination of ceftazidime and tazobactam in injectable powder. Methods Chromatography was carried out on Zorbax 300SB-C18 column using a mixture of methanol and aqueous solution of phosphate buffer (pH = 5.6) as mobile phase. The UV detection wavelength was 220 nm. Results The linear ranges of ceftazidime and tazobactam were 0.62-631.8 μg·mL^-1 and 0.66-677.50μg·mL^-1, respectively. The average recoveries were 98.8%-101.4% for ceftazidime, and 99.1%-100.2% for tazobactam. The RSD values of inter-day and intra-day assays were lower than 1.5% for ceftazidime and 2.6% for tazobactam. Conclusion This method is reproducible, simple, precise, and rapid for the quality control of ceftazidime and tazobactam in injectable powder.

Key words: ceftazidime, ceftazidime, tazobactam, tazobactam, RP-HPLC, RP-HPLC

CLC Number:

R917.01

R917.795

Supporting:

MENG Xiang-ming, MENG Zhi-yun, ZHANG Liang, DOU Gui-fang^*. Simultaneous Determination of Ceftazidime and Tazobactam in Injectable Powder by Reversed-Phase High Performance Liquid Chromatography[J]. .

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Simultaneous Determination of Ceftazidime and Tazobactam in Injectable Powder by Reversed-Phase High Performance Liquid Chromatography

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