Neuroprotective Effects of Bushen Decoction Against Glutamate-Induced Neurotoxicity in PC12 Cells

Abstract

Abstract: Aim The enhanced effect of Bushen (Kidney-tonifying) decoction (BS) on cultured PC12 cell proliferation and its antagonistic action on neurotoxicity induced by glutamate were investigated by serum pharmacological method of the Chinese material medica (CMM) in vitro. Methods The effect of BS on cultured PC12 cell activity and its antagonistic action on neurotoxicity induced by glutamate was observed by MTT method. Flow cytometry and fluorescence microscope techniques were employed to observe the antagonistic effect of BS on early period apoptosis of PC12 cells induced by glutamate. Results The serum with BS was able to enhance activity of PC12 cells and exert antagonistic effect on glutamate-induced neurotoxicity. Meanwhile, these beneficial effects produced by BS were found to be the strongest in 20% concentration of in serum BS. Moreover, it can inhibit apoptosis of PC12 cells induced by glutamate, which occurs in the early period. Conclusion BS may exert a potential neuroprotective effect.

Key words: Bushen decoction, Bushen decoction, neuroprotective effect, neuroprotective effect, apoptosis, apoptosis, pharmacology Chinese material medica, pharmacology Chinese material medica, PC12 cells, PC12 cells

CLC Number:

R285

R285.5

Supporting:

HE Wen-bin, ZHANG Jun-long, CHEN Nai-hong, ZHANG ling, ZHU Hai-bo^*. Neuroprotective Effects of Bushen Decoction Against Glutamate-Induced Neurotoxicity in PC12 Cells[J]. .

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