Protective effects of Polygonatum sibiricum against CCl4-induced acute liver injury in rats through oxidative stress and mitochondrial apoptotic pathways

doi:10.5246/jcps.2021.04.025

Abstract

Abstract:

In the present study, we aimed to investigate the hepatoprotective effect of Chinese herbal medicine Polygonatum sibiricum (PS). In this study, a rat acute liver injury (ALI) model was established by a single intraperitoneal injection of 50% CCl₄oil solution, and the rats were treated intragastrically with Polygonatum sibiricum aqueous extract (PSAE). The results showed that PSAE significantly decreased the serum levels of ALT, AST and ALP, increased the activities of glutathione (GSH) and superoxide dismutase (SOD), decreased malondialdehyde (MDA) activity in hepatic tissue, and decreased the reactive oxygen species (ROS) level in hepatocytes. The expressions of Nrf2, NQO-1, HO-1, Bcl-2, Bcl-xL mRNA, and HO-1 proteins were elevated, and the expression of p53 mRNA was decreased. In conclusion, PSAE exerted a powerful protective action against CCl₄-induced ALI in rats via effectively regulating the expressions of Nrf2-Keap1-ARE related genes and proteins, and inhibiting hepatocyte apoptosis. These outcomes provided evidence that PS had apparent hepatoprotective effect.

Key words: Polygonatum sibiricum, CCl4, Rat, Acute liver injury, Oxidative stress, Apoptosis

Supporting:

Chunyang Han, Taotao Sun, Guangtai Fan, Yawei Liu, Cuiyan Liu. Protective effects of Polygonatum sibiricum against CCl4-induced acute liver injury in rats through oxidative stress and mitochondrial apoptotic pathways[J]. Journal of Chinese Pharmaceutical Sciences, 2021, 30(4): 306-318.

Figures/Tables 7

References 43

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