Simultaneous Determination of Clonidine Hydrochloride,Hydrochlorothiazide, and Rutin in Zhenju Jiangya Tablet by Capillary Electrophoresis

Abstract

Abstract:

Aim To develop a method for the determination of three drug components: clonidine hydrochloride, hydrochlorothiazide and rutin in Zhenju Jiangya tablet. Methods An uncoated capillary tube was used to analyze under 20 kV voltage at 20 ºC. The samples were introduced into the capillary tube by hydrodynamic mode applying 50 kPa for 5 s and detected at 210 nm. Results The linear ranges of clonidine hydrochloride,hydrochlorothiazide, and rutin were 10 μg·mL^-1-100 μg·mL^-1, 30 μg·mL^-1-300 μg·mL^-1, and 30 μg·mL^-1-300 μg·mL^-1, respectively. Inter-day and intra-day RSD were all below 10.5%. The recoveries were 94.96% for clonidine hydrochloride, 84.45% for hydrochlorothiazide, and 89.88% for rutin. Conclusion Clonidine hydrochloride,hydrochlorothiazide, and rutin are baseline separated. The method is simple and rapid for simultaneous determination of the three drug components in Zhenju Jiangya tablet.

Key words: capillary electrophoresis, capillary electrophoresis, clonidine hydrochloride, clonidine hydrochloride, hydrochlorothiazide, hydrochlorothiazide, rutin, rutin, Zhenju Jiangya tablet, Zhenju Jiangya tablet

CLC Number:

R917.4

R944.4

Supporting:

SU Sheng-min, YU Yun-qiu^*. Simultaneous Determination of Clonidine Hydrochloride,Hydrochlorothiazide, and Rutin in Zhenju Jiangya Tablet by Capillary Electrophoresis[J]. .

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Simultaneous Determination of Clonidine Hydrochloride,Hydrochlorothiazide, and Rutin in Zhenju Jiangya Tablet by Capillary Electrophoresis

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