Urinary metabolomics analysis of the anti-depressive effects of Hemerocallis citrina extracts in a simulated microgravity-induced rat model of depression

doi:10.5246/jcps.2020.03.015

Abstract

Abstract:

We investigated the antidepressant-like activity of Hemerocallis citrine Baroni extract (HCE) in a simulated microgravity (SMG)-induced rat model of depression using a metabolomics method. A rat model, generated via 14 d of SMG induction, was validated from the reduced sucrose preference and the enhanced immobility time in the forced swimming test. HCE and paroxetine reversed certain metabolite profiles. Anti-depressant effects of HCE might involve the regulation of several metabolic pathways, such as phenylalanine, glutamic acid, and tryptophan metabolism and changes in energy metabolism. 5-Hydroxytryptophan, hippuric acid, phenylacetylglycine, citric acid, 3-hydroxykynurenine, cyclic AMP, and L-DOPA profiles were altered upon HCE and paroxetine administration. Furthermore, glutamic acid was only regulated in the HCE group, while xanthurenic acid and deoxyuridine were reversed in the positive group, suggesting differences in the mechanisms between the positive drugs and HCE in improving glutamic acid metabolism. This study provided a theoretical foundation for the application of HCE in depression therapy.

Key words: Hemerocallis citrina, Anti-depressive, Ultra-performance liquid chromatography quadrupole time-of-flight mass spectrometry, Ultra-high performance liquid chromatography coupled to triple-quadrupole mass spectrometry, Metabolomics

CLC Number:

R965

Supporting:

Teng Xu, Yan Wang, Cong Lu, Li Feng, Linxi Fan, Jing Sun, Bei Fan, Qiong Wang, Xinmin Liu, Fengzhong Wang. Urinary metabolomics analysis of the anti-depressive effects of Hemerocallis citrina extracts in a simulated microgravity-induced rat model of depression[J]. Journal of Chinese Pharmaceutical Sciences, 2020, 29(3): 176-191.

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