Application of avidin-phospholipid vesicle complex as the stationary phase for chiral separation in open-tubular capillary electrochromatography

doi:10.5246/jcps.2017.12.097

Abstract

Abstract:

In the present study, we developed a novel open-tubular capillary electrochromatographic method using avidin-phospholipidvesicle complex as the stationary phase for chiral separation of mexiletine hydrochloride. The avidin immobilized on the phospholipid vesicle consisting of 1,2-dipalmitoyl-sn-glycero-3-phosphoethanolamine (DPPE) and L-α-phosphatidyl-L-serine (PS) (80:20, mg%) was coated in the capillary. The homogeneity and separation performance of the coating were evaluated in terms of phospholipid vesicle characterization and the resolution of D,L-Tryptophan. As for mexiletine hydrochloride, four vital parameters affecting the separation efficiency of coating capillary, including buffer type, buffer pH, buffer concentration and the applied voltage, were studied in detail. Under the optimum conditions, the enantiomers could be separated well with good resolution. All the satisfactory results indicated that this method using avidin-phospholipid vesicle complex as the stationary phase was suitable and feasible, which had great potential in pharmaceutical separation of enantiomers.

Key words: Avidin, OTCEC, Chiral separation, Phospholipid vesicle, Mexiletine hydrochloride

CLC Number:

R917

Supporting:

Ye Gao, Bailing Qiu, Qihui Liang, Di Wu, Yu Song, Nanyin Han. Application of avidin-phospholipid vesicle complex as the stationary phase for chiral separation in open-tubular capillary electrochromatography[J]. Journal of Chinese Pharmaceutical Sciences, 2017, 26(12): 872-880.

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