Simultaneous determination of four triterpenoid saponins and two liganoids in the roots of Ilex pubescens by RP-HPLC-DAD

doi:10.5246/jcps.2014.12.110

Journal of Chinese Pharmaceutical Sciences ›› 2014, Vol. 23 ›› Issue (12): 866-872.DOI: 10.5246/jcps.2014.12.110

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Simultaneous determination of four triterpenoid saponins and two liganoids in the roots of Ilex pubescens by RP-HPLC-DAD

Yuan Zhou¹, Ning Li¹, Jiayu Zhang², Yong Jiang¹, Pengfei Tu^1,3*

1. State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University Health Science Center, Beijing 100191, China
2. Center of Scientific Experiment, Beijing University of Chinese Medicine, Beijing 100029, China
3. Modern Research Center for Traditional Chinese Medicine, Beijing University of Chinese Medicine, Beijing 100029, China

Received:2014-08-20 Revised:2014-09-24 Online:2014-12-25 Published:2014-10-11
Contact: Tel./Fax: 86-10-82802750
Supported by:
Research Fund for the Doctoral Program of Higher Education of China (Grant No. 20110001130003); the National Key Technology R&D Program “New Drug Innovation” of China (Grant No. 2012ZX09301002-002-002 and 2012ZX09304-005).

Abstract

Abstract:

A reversed-phase high performance liquid chromatography (RP-HPLC) method with diode array detector (DAD) has been established to simultaneously determine six bioactive compounds in the roots of Ilex pubescens, namely tortoside A, (+)-syringaresinol, ilexsaponin B₃, ilexsaponin A₁, ilexsaponin B₁, ilexgenin A. The RP-HPLC assay was performed on a reversed-phase C₁₈ column with a gradient elution. The mobile phase consisted of acetonitrile and water containing 0.1% (v/v) phosphoric acid. The flow rate was 1.0 mL/min and the detection wavelength was set at 210 nm. The six marker constituents were separated well with good linearity (r²>0.9996), precision, stability and repeatability. The overall recoveries were in the range of 99.00%–104.52%. Cluster analysis was employed to analyze 15 batches of samples. The result indicated this method provide an efficient way to perform quality control as well as a scientific rationale for the Geo-authentication of I. pubescens.

Key words: RP-HPLC-DAD, Aquifoliaceae, Ilex pubescens, Triterpenoid saponin, Liganoid, Cluster analysis

CLC Number:

R284

Supporting:

Yuan Zhou, Ning Li, Jiayu Zhang, Yong Jiang, Pengfei Tu. Simultaneous determination of four triterpenoid saponins and two liganoids in the roots of Ilex pubescens by RP-HPLC-DAD[J]. Journal of Chinese Pharmaceutical Sciences, 2014, 23(12): 866-872.

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Simultaneous determination of four triterpenoid saponins and two liganoids in the roots of Ilex pubescens by RP-HPLC-DAD

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