Characterization of chemical constituents and in vivo metabolites of Kai-Xin-San prescription by HPLC/DAD/ESI-MSn

doi:10.5246/jcps.2012.06.072

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Characterization of chemical constituents and in vivo metabolites of Kai-Xin-San prescription by HPLC/DAD/ESI-MSn

Chunfang Liu, Wenzhi Yang, Kedi Liu, Xue Qiao, Yang Liu, Yanyan Jiang, Renbing Shi*, Tao Bo, Xiuwei Yang, Min Ye^*

1. State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University Health Science Center, Beijing 100191, China
2. School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 100102, China
3. Agilent Technologies, Inc., 3 North Wangjing Road, Beijing 100102, China

Received:2012-06-04 Revised:2012-08-15 Online:2012-10-25 Published:2012-10-25
Contact: Renbing Shi*, Min Ye*

Abstract

Abstract:

This work aims to elucidate the chemical constituents of Kai-Xin-San (KXS) and its metabolites in rat plasma. KXS extracts were separated on an Agilent HPLC SB-C₁₈ column, analyzed by ion-trap tandem mass spectrometry and high-accuracy qTOF mass spectrometry in negative ion mode. A total of 39 compounds, including 11 ginsenosides, 14 Polygala saponins, 5 sucrose esters, 8 oligosaccharide esters and 1 xanthone were characterized from KXS. Fifteen of them were confirmed by reference standards. No constituents were detected from Poria or Acori Tatarinowii Rhizoma. After oral administration of KXS (7 g/kg), 10 ginsenosides and 18 Polygala compounds were detected in rat plasma. This study indicates that ginseng saponins, Polygala saponins and saccharide esters could be the major effective components of KXS prescription.

Key words: Kai-Xin-San, Chemical analysis, Metabolites identification, HPLC/DAD/ESI-MSn

CLC Number:

R931

Supporting:

Chunfang Liu, Wenzhi Yang, Kedi Liu, Xue Qiao, Yang Liu, Yanyan Jiang, Renbing Shi*, Tao Bo, Xiuwei Yang, Min Ye^*. Characterization of chemical constituents and in vivo metabolites of Kai-Xin-San prescription by HPLC/DAD/ESI-MSn [J]. , DOI: 10.5246/jcps.2012.06.072.

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Characterization of chemical constituents and in vivo metabolites of Kai-Xin-San prescription by HPLC/DAD/ESI-MSn

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