Abstract: Aim The oxymatrine phospholipid complexs were prepared, and its activity against hepatitis B virus in vitro were studied. Methods Using tetrahydrofuran as a reaction medium, oxymatrine and phospholipids were resolved into the medium. Oxymatrine phospholipid complexs were prepared. The toxicity measurements and inhibitory effect on HBsAg, HBeAg, and HBVDNA of oxymatrine phospholipid complex in 2.2.15 cells were studied respectively. Results The content of oxymatrine in the phospholipids complexs prepared was 24.86% (W/W). The TCO of the oxymatrine phospholipid complexs was 250 μmol×L^–1. The inhibitory effect of HBsAg, HBeAg, HBV-DNA of 2.2.15 cells treated by oxymatrine phospholipid complex were higher than those of the oxymatrine. Conclusion Oxymatrine phospholipid complex can have stronger effective activity against hepatitis B virus compared with oxymatrine. So oxymatrine phospholipid complexs were showing its potential antiviral activity in hepatitis B treatment.

Key words: Oxymatrine phospholipid complex, Oxymatrine phospholipid complex, 2.2.15 Cell line, 2.2.15 Cell line, HBV-DNA, HBV-DNA